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A first-in-human, double-blind, placebo-controlled phase 1b/2a trial evaluated nebulized three-phage cocktail BX004-A in nine adults with cystic fibrosis and chronic Pseudomonas aeruginosa infection. The trial met its primary safety and tol

In mouse models of acute Pseudomonas aeruginosa pneumonia, depleting alveolar macrophages shortened the survival of adult male mice without raising lung bacterial load, yet lung P. aeruginosa levels were significantly lower in macrophage-de

A PNAS study isolated 66 phage escape mutants against 13 plasmid-encoded lactococcal antiphage systems and found 15 mutated genes, and proposes that the Audmula system modifies the host cell wall, delaying lysis and release of progeny phage

Rand and colleagues present Phage DisCo (phage discovery by co-culture) in mSystems: bacterial strains that are isogenic except for fluorescent tags and one perturbation expected to affect phage infection are co-cultured on the same screeni

Review by Feng et al. in Precision Clinical Medicine on the human gut virome, 90%-95% of which is phages. It examines how the virome develops and how diet, environment, host genetics and immunity shape it, its associations with various dise

Adams and colleagues report in Nature Microbiology that the two genetic signatures defining the West African–South American (WASA) lineage of Vibrio cholerae, responsible for the 1991-2001 Latin American cholera epidemic, encode multiple an

She et al. retrieved 5,178 viral sequences encoding 12 Salmonella enterica virulence genes from the IMG/VR database and compared them with 466,136 S. enterica genomes from EnteroBase. Virulence genes including iacP, mgtB, misL and fliC were

Byers et al. report that telomere phages, which replicate their genomes as linear extrachromosomal elements, are widely distributed in bacteria and highly prevalent in Klebsiella species. In phage-host cells only a small set of phage protei

Ortiz de Ora and colleagues created Phollow, a live-imaging approach that tracks phage replication and spread in situ at single-virion resolution by marking virions with distinct fluorescent proteins as they assemble in newly infected cells

A Cell paper reports the complete structure and atomic model of mycobacteriophage Bxb1, a virus of Mycobacterium smegmatis, including the capsid, tail tube and tail-tip assembly. Cryoelectron tomography of phage bound to M. smegmatis shows

A review of recent advances in using bacteriophages to treat the most common bacterial infectious diseases of cultured fish, shellfish and crustaceans. It concludes that the documented research supports phage therapy in aquaculture but that

This review surveys lytic bacteriophages and related approaches being studied against antibiotic-resistant Klebsiella pneumoniae. Bacteriophage therapy: a possible alternative therapy against antibiotic-resistant strains of Klebsiella pneum

Bacteriophages of the Chimalliviridae family are shown to use two successive compartments during infection: a membrane-bound early phage infection (EPI) vesicle that encloses the injected genome and expresses early genes, and the protein-la

Saavedra, Silva-Santos, Duarte and Azevedo describe in Journal of Chromatography A a purification workflow for bacteriophage preparations built around anion-exchange chromatography, using phage T4 and E. coli K12 as models. Treating with al

In vitro, curcumin (400 ug/mL) combined with the newly isolated phage vB_AbaSI_1 reduced multidrug-resistant A. baumannii to undetectable levels within 1 hour, and a double dose extended this to 5 hours, whereas phage alone rebounded within

Wrapping individual phage-infected bacteria in a polymeric nanoscale coating preserves the vitality of the phages released on lysis, which otherwise have severely degraded tail proteins. In mice with bacterially induced enteritis and associ

A questionnaire study of 1,098 people in Poland, from the Hirszfeld Institute of Immunology and Experimental Therapy and colleagues, found antibiotic-resistance awareness above 90% but awareness of phage therapy between 8.9% and 39.7% depen

George Wright, Slawomir Michniewski, Eleanor Jameson and Fayyaz ul Amir Afsar Minhas describe DepoRanker, a machine-learning algorithm that ranks proteins by their likelihood of being phage depolymerases targeting Klebsiella capsular polysa

Indigo carmine, an FDA-approved food additive, selectively inactivated DNA phages (P001, T4, T1, T7, lambda) but not the RNA phage MS2, by binding double-stranded DNA and causing genome ejection from the capsid; under optimal conditions (50

A binary-interaction screen identified coelichelin, a siderophore secreted by Streptomyces sp., as a metabolite that sensitizes its soil competitor Bacillus subtilis to lytic phages (SPO1, SP10, SP50, Goe2) by sequestering iron and preventi