Search

This review surveys phage genomics, phage–host interactions, and medical and biotechnological applications, including mechanisms and therapeutic uses in infections involving virus–bacteria interactions. www.mdpi.com

PHIStruct is a multilayer perceptron that uses SaProt structure-aware embeddings of receptor-binding proteins to predict hosts among the ESKAPEE genera. The study provides its experimental data and source code on GitHub. Oxford Academic / P

Metabolites released from intact, phage-infected Synechococcus strongly attracted the marine bacteria Vibrio alginolyticus and Pseudoalteromonas haloplanktis, especially in early infection, while uninfected cyanobacteria did not. 5'-deoxyad

Using a dataset of interactions between 403 Escherichia strains and 96 phages, researchers predicted strain-level phage-bacteria outcomes from genomic data alone (AUROC 86%) and found that adsorption factors explain most interactions while

Researchers at Queen's University Belfast and University College Dublin found that combining cold plasma-activated water with phage vB_PmiS_PM-CJR reduced biofilm-embedded Proteus mirabilis cells by about 4 log, as a possible option for cat

Querying 34 toothbrush and 92 showerhead metagenomes, researchers identified 22 complete, 232 high-quality and 362 medium-quality viral OTUs, with viral richness correlating with bacterial richness. Low sequence similarity to references and

A hydrogel loaded with a phage cocktail and vancomycin gave a 99.72% reduction in MRSA biofilm in vitro and maintained activity for eight days. In vivo, the co-delivery hydrogel with systemic antibiotic reduced bacterial load by 0.99 log10

This review describes the computational tools used by SEA-PHAGES and PHIRE to analyze phage genomes, extract prophages, group related genes into phamilies, and visualize genome mosaicism. The tools are freely available to the phage communit

“We created a MASH database for rapid searching of closely related phages, to then be compared with BLASTn to compute intergenomic similarity between bacteriophages.” The authors report that taxmyPHAGE is available as a conda repository and

A bacterial antiphage system made of a ubiquitin-like protein, E1 and E2 conjugating enzymes and a deubiquitinase attaches the ubiquitin-like protein to the phage central tail fibre during infection. Infected cells release tailless particle

A portable library of solid tablets, each encapsulating one phage with luciferin and luciferase stabilized in a pullulan-trehalose sugar matrix, detects phage-mediated ATP release as light when bacterial cells burst. The platform identified

The helix-turn-helix regulator Aca2 represses phage anti-CRISPR synthesis transcriptionally by binding DNA and also inhibits translation of the mRNAs by binding conserved RNA stem-loops and blocking ribosome access; a cryo-EM structure of t

The study predicted 76 INPHARED genomes and 712 Unified Human Gut Virome Catalogue vOTUs that repurpose a stop codon to encode an amino acid, and re-annotated them with modified Pharokka-gv and Prokka-gv tools. For sequences predicted to re

PharaCon applies conditional BERT to phage identification, incorporating labels during pre-training and fine-tuning. The authors evaluated the framework on simulated sequences and real metagenomic contigs. bioRxiv

A retrospective observational analysis of the first 100 consecutive cases of personalized phage therapy facilitated by a Belgian consortium in 35 hospitals across 12 countries (2008-2022) reports clinical improvement in 77.2% and eradicatio

Braunstein and colleagues report in The Veterinary Quarterly the personalized phage-antibiotic treatment of a cat with a multidrug-resistant Pseudomonas aeruginosa implant-associated infection after arthrodesis surgery: a tailored anti-P. a

Fluorescence in situ hybridization showed that up to 19% of SAR11 (Pelagibacterales) cells were phage-infected during a phytoplankton bloom, coinciding with a roughly 90% drop in SAR11 abundance within 5 days. A fraction of infected cells h

A human oral Saccharibacteria isolate (TM7x) protected its host Schaalia odontolytica XH001 from lytic phage LC001 during surface-associated growth, with down-regulation of a cell-wall polysaccharide gene cluster that affects phage binding.

Researchers at University Medicine Rostock delivered synthetic mRNA encoding the Cpl-1 phage endolysin into three human cell lines (HEK293T, A549, HepG2), which produced endolysin with activity against Streptococcus pneumoniae. Adding a hum

Integrated resonant photonic crystal cavities in silicon acted as optical nanotweezers that differentiated a single bacterium from a single phage without labeling or surface bioreceptors, and distinguished phages across phenotypically disti