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A case series from the Israeli Phage Therapy Center describes phage PASA16, provided pro bono by Adaptive Phage Therapeutics, given intravenously, locally or topically with antibiotics to 16 patients with non-resolving Pseudomonas aeruginos

Tulane University researchers used inducible CRISPR interference to manipulate 18 E. coli BL21 genes and identify effectors of phage T7 propagation, then modified the bacterial lysate used for cell-free bacteriophage synthesis accordingly.

ETH Zurich researchers genetically engineered two virulent phages per uropathogen (E. coli, Enterococcus spp., Klebsiella spp.) to express a nanoluciferase reporter on infection and tested the bioluminescence assay on 206 patient urine samp

Nature Communications case report of a 41-year-old man with Kartagener syndrome and a life-threatening chronic multidrug-resistant Pseudomonas aeruginosa lung infection who was treated successfully with iterative aerosolized phage treatment

Streptomyces lividans, a model filamentous Gram-positive bacterium with conserved contractile-injection-system gene clusters, makes phage tail-like particles (SLPs) that stay in the cytoplasm instead of being released. Without SLPs, aerial

Nature Communications paper that builds artificial viral vectors (AVVs) from bacteriophage T4 components: a 120 × 86 nm capsid shell that holds 171-Kbp DNA and thousands of protein copies is loaded with DNAs, proteins, RNAs and ribonucleopr

PLOS ONE survey (N = 787, Prolific) of UK public awareness and acceptance of phage therapy found moderate acceptance, a mean likelihood of 4.71 on a 1-7 scale. Priming participants to think about novel medicines and antibiotic resistance ra

Nature Communications paper using cryo-electron microscopy of short Ff-derived nanorods to determine a structure of a filamentous virus (f1, fd or M13 phages) including the tips. Combined with mutagenesis, it identifies phage domains import

Nature Biotechnology paper on SNIPR001: from a library of 162 wild-type phages, eight with broad E. coli coverage were selected and engineered with tail fibers and CRISPR–Cas machinery to target E. coli. A combination of the four most compl

University of Warwick and Cytiva authors show that poly(acrylic acid) prevents phage-induced killing of bacterial hosts and phage replication without affecting induction of recombinant protein expression, and was more active than poly(metha

iPHoP, from the DOE Joint Genome Institute, is a two-step framework that integrates multiple methods to predict host taxonomy at the genus rank for viruses infecting bacteria and archaea while retaining a low false discovery rate. Applied t

A bioRxiv preprint reports that highly purified phage T4 was rapidly internalized in vitro by A549 lung epithelial cells, MDCK-I kidney cells and mouse bone marrow-derived macrophages without activating TLR9 or cGAS-STING inflammatory pathw

Live-cell fluorescence microscopy shows that mycobacteriophage infection of Mycobacterium smegmatis depends on the host nucleoid-associated protein Lsr2, which reorganizes away from host replication foci to set up zones of phage DNA replica

The study surveyed 19 metagenomic phage-detection tools and benchmarked nine that could be installed and run at scale, comparing performance across fragmented genomes, simulated metagenomes, and real samples. Evaluation of computational pha

University of Exeter authors show, using Pseudomonas aeruginosa PA14 and phage DMS3vir, that antibiotics targeting protein translation raise CRISPR-Cas immunity against phages carrying anti-CRISPR (acr) genes by interfering with production

University of Nebraska-Lincoln authors show that small protists can consume viruses and grow and divide given only viruses to eat; the ciliate Halteria sp. foraging on chloroviruses shows dynamics similar to other microbial trophic interact

McMaster University researchers (Lei Tian, Zeinab Hosseinidoust and colleagues) crosslinked half a million filamentous phages into each soft microgel, producing over 35,000 microgels per square centimetre of film template, a 13-billion-phag

Nature paper (Laughlin et al.) reporting that the nucleus-like shell with which jumbo phages protect their replicating genomes from host defences assembles primarily from one protein, named chimallin (ChmA). Cryo-electron tomography in infe

INHERIT combines database-based and alignment-free phage identification using deep representation learning. It achieved an F1 score of 0.9932 on a third-party benchmark; pre-training on two species separately improved prediction. bioRxiv

A novel strain of Fusarium graminearum virus 1 (FgV1-SD4), a virus of the fungus rather than a bacteriophage, was the only mycovirus found in an F. graminearum isolate with significantly reduced virulence on wheat. No deoxynivalenol (DON, v