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Eleanor Rand (Harvard Medical School) and colleagues published a new paper on a targeted phage discovery method called Phage DisCo, a co-culture-based phage screening technique. Using isogenic, fluorescently tagged bacterial strains, they c

Amit Rimon (Hebrew University of Jerusalem) and colleagues published a new paper on comparing methods for assessing phage activity against biofilms, showing several promising techniques including calorimetry, extracellular ATP measurement,

Su Jin Jo (Seoul National University) and colleagues published a paper on a new model they've developed for standardizing and optimizing an agar plate-based method for phage production based on plaque size and inoculum concentration.

Siti Adil (La Trobe University) and colleagues published a new phage purification methods comparison paper, showing Triton X-100 was most effective for endotoxin removal and phage recovery compared to CsCl ultracentrifugation and endotoxin

Jyot Antani (Yale University) and colleagues developed a microscopy-based technique to measure phage attachment to bacterial cells. This method provides an attractive alternative to the (literally) century-old Phage Adsorption Assay because

Silvia Würstle and colleagues at the Center for Phage Biology & Therapy at Yale University published a paper on their optimized pipeline for emergency phage therapy against P. aeruginosa. Their pipeline includes evaluation of useful evolved

Justin Boeckman (Center for Phage Technology, Texas) and colleagues published a protocol detailing methods for phage DNA extraction, genome assembly, and genome closure.

From @scientiff via Twitter: Hey #scitwitter! I’m troubleshooting some #phage DNA extraction woes with our undergrads. We use phenol-chloroform-isoamyl and phase-lock gels for the process but we’re still getting high 260/230 after elution.

Elizabeth Burgener (Stanford University) and colleagues published a new preprint on methods for extracting and detecting phage DNA from CF patient sputum.

Elizabeth Burgener (Stanford University) and colleagues published a new preprint on methods for extracting and detecting phage DNA from CF patient sputum.

Important question: if I had a student who wanted to make an undomesticated bacteriophage mutant that could not make plaques, other than conditionally, is there a way other than bashing plaque isolates at two temperatures after random mutag

Important question: if I had a student who wanted to make an undomesticated bacteriophage mutant that could not make plaques, other than conditionally, is there a way other than bashing plaque isolates at two temperatures after random mutag

When doing a PEG precipitation of phages, some people (like me) use a PEG stock solution (20-30% w/v PEG in NaCl), whereas other people add the PEG powder directly to the sample. What are the pros and cons? — @EvelienAdri Check out the Twi

When doing a PEG precipitation of phages, some people (like me) use a PEG stock solution (20-30% w/v PEG in NaCl), whereas other people add the PEG powder directly to the sample. What are the pros and cons? — @EvelienAdri Check out the Twi

Ushanandini Mohanraj (University of Helsinki) and colleagues have published a paper showing a screen they've developed to identify hypothetical phage proteins with antibacterial activity. They've used it to identify four Yersinia phage prot

Ushanandini Mohanraj (University of Helsinki) and colleagues have published a paper showing a screen they've developed to identify hypothetical phage proteins with antibacterial activity. They've used it to identify four Yersinia phage prot

Hans Duyvejonck and colleagues at Ghent University and the Queen Astrid Military Hospital in Belgium have published a paper evaluating the use of qPCR to quantify 5 phages within a cocktail. They compared qPCR to plaque assays, and showed t

Hans Duyvejonck and colleagues at Ghent University and the Queen Astrid Military Hospital in Belgium have published a paper evaluating the use of qPCR to quantify 5 phages within a cocktail. They compared qPCR to plaque assays, and showed t

Question: Hello phage people, does anyone have an improved method to physically remove #phages from liquid broth? Thank you! — @PetrovicFabijan A subset of the answers to date: - We use Amicon filters in my lab to concentrate phage and we

Question: Hello phage people, does anyone have an improved method to physically remove #phages from liquid broth? Thank you! — @PetrovicFabijan A subset of the answers to date: - We use Amicon filters in my lab to concentrate phage and we