Search

Tired of doing plaque assays? Check out the Bollyky lab’s new method to count phages and assess their bioactivity using a DLS Particle Size Analyzer, now published in PNAS. Great tweet thread here!

Hello Phage People, Does anyone use Phage DNA Isolation Kit from Norgen Biotek? I'm using this kit and trying to increase the DNA concentration. I already did all the additional steps but does anyone have any tricks for increasing concentra

Craig MacLean on Twitter: "Quick question: does anybody know of fluorescent dyes that can be used to stain phage? Want to measure ability of phage to adsorb to bacterial cells they cannot infect."

Jutta Kasurinen (University of Helsinki) and colleagues published a paper in Viruses on a new method for screening for phage-encoded toxic proteins using a Next Generation Sequencing-based assay.

Prisca Perlemoine (University Grenoble Alpes, France) and colleagues published a new paper in PLOS ONE on phage susceptibility testing and infectious titer determination through wide-field lensless monitoring of phage plaque growth.

Abbas Soleimani-Delfan (University of Isfahan) and colleagues published a new paper in Journal of Virological Methods showing a rapid new method for phage genomic DNA extraction.

Holger Buchholz (University of Exeter, UK) and colleagues published a new paper in The ISME Journal showing a high-throughput method for isolating virus-host model systems for fastidious heterotrophic bacteria. Using this method, 117 new ph

BCM Tailor labs has posted a video tutorial on how to do a quick phage precipitation. In this video you will learn how to precipitate your phage in 10 to 15 minutes. This is in order to concentrate your phage from a solution. This can be mo

The TAILOR team at Baylor College of Medicine now has a YouTube channel! Check out their recent one, a recorded talk on AMR and phage therapy by one of TAILOR's founders, Dr. Anthony Maresso, called Chasing Change: A moving pandemic, an ada

Shannon Miller (Broad Institute) and colleagues published a new paper in Nature Protocols on phage-assisted continuous and non-continuous evolution. This method allows directed evolution to occur much faster (100 rounds of evolution in 2 we

Katie Bodner (Stanford University) and colleagues published a new paper in STAR Protocols showing how to engineer phages for live-cell imaging of prophage induction inside mammalian cells.

The use and selection of excipients are critical to stabilize phages in solid formulations. Yajie Zhang (University of Texas at Austin) and colleagues have reviewed the current classes of phage excipients, outstanding challenges with phage

Here's a streamlined new method for producing phages (and removing endotoxin) for personalized phage therapy, published in Nature Protocols by Tiffany Luong (San Diego State University) and colleagues.

Hello, #phage tweeps! I have a question. Suppose you have a bunch of phage MAGs, each composed of more than 1 contig: how do you calculate their completeness? I have a couple of ideas, but I still need to evaluate their feasibility. - @Mich

Dragica Damnjanovic (UNSW Sydney) and colleagues published a paper in BMC Microbiology on phage genotyping using repetitive-PCR, a rapid and inexpensive method to preliminarily screen phage isolates prior to selecting them for future studie

Got phage protocols, or wish you did? The community can probably help! Here's an example from Twitter: "Hey, I'm working on phages and need to extract phage dna for characterization. Would you recommend Zymo kit for extraction? If not, whi

Maciej Konopacki (West Pomeranian University of Technology, Poland) and colleagues published a new paper in Biochemical Engineering Journal describing PhageScore, a new approach for comparative evaluation of phage lytic activity.

Maciej Konopacki (West Pomeranian University of Technology, Poland) and colleagues published a new paper in Biochemical Engineering Journal describing PhageScore, a new approach for comparative evaluation of phage lytic activity.