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A high-throughput droplet microfluidics platform co-encapsulates individual phages and bacteria to quantify single phage infection events, with control over exposure time and the phage-to-bacteria ratio. It measures lysis events and lysis k

The study links temperate-phage capsule specificity with diverse receptor-binding proteins, including classical depolymerases and SGNH-domain hydrolases predicted to deacetylate polysaccharides. The team also shared English and Polish comic

Large-scale comparative cell biology and biochemistry of 16 bacterial nuclease-NTPase antiphage defence systems shows that protein-protein complex formation is a shared feature. The nucleases of the Pa AbpAB, Bt Hachiman and Ec PD-T4-8 syst

A perspective arguing that traditional bacteriophage methods measure population averages and miss variation between individual particles. It advocates optical microscopy and flow cytometry approaches that can reveal phenotypic heterogeneity

Terlizzi and colleagues report a systematic review in the International Journal of Infectious Diseases of phage therapy in cystic fibrosis: 19 studies covering 51 patients and 52 phage treatment courses, mostly against Pseudomonas aeruginos

VirHost Hunter uses phage tails and lysins with protein language models and vision transformers to assign hosts without requiring complete phage genomes. High-resolution phage-host assignment through key proteins using large language models

Godsil, Wei and Meeske report in Nature Microbiology that the Listeria seeligeri type VI-A CRISPR system restricts the lytic cycle of temperate phages but tolerates prophage acquisition and interferes with prophage induction through a non-a

Intralytix, Inc. has been awarded a Maryland Manufacturing 4.0 grant to modernize phage manufacturing. It will expand its PhageEngine platform by integrating in silico omics data and building a digital twin to speed process optimization. In

A Bioengineering & Translational Medicine study from the University of Sydney spray-dried phage PEV1 with polyvinylpyrrolidone (PVP) grades K15 to K100 and stored it for 180 days at 4, 22 and 40 °C and 15 to 53% relative humidity. At 33% re

Damgaard and colleagues report in Communications Medicine that Bacteroides fragilis isolates from colorectal cancer (CRC) patients are infected with specific Caudoviricetes prophages significantly more often than controls, a finding made by

Patel and colleagues describe in Nature a 164-residue prophage-encoded defence protein, ring interacting pore 1 (Rip1), that is activated by the portal or small terminase proteins of infecting phages, oligomeric ring-shaped complexes essent

An automated screening platform combining liquid-handling robotics, standardized phenotypic assays and computer-vision enumeration screens large phage collections against clinical bacterial strain panels and has assessed more than 3.8 milli

HepS, encoded by the Gifsy-1 prophage in Salmonella enterica serovar Typhimurium, detects infection by Siphoviridae phages and cleaves tRNA anticodon loops to halt phage replication. Gifsy prophages evade self-targeting through a tail-tip v

Sikkema and colleagues describe in PNAS an engineering platform for the phiKMV-like Pseudomonas aeruginosa phage 41S1 built on one-pot High Complexity Golden Gate assembly (HC-GGA): the genome is divided into synthesizable DNA fragments tha

P2D1 tailocin from Dickeya dadantii was active against nine Pseudomonas strains among 480 soil and rhizosphere isolates. Six susceptible, nonpathogenic strains mitigated Dickeya-induced soft rot on potato; three related P. tensinigenes isol

A PLOS Biology study by Huss, Chitboonthavisuk, Meger and colleagues reports the dynamics between T7 bacteriophage and Escherichia coli aboard the International Space Station: phage activity was initially delayed in microgravity but ultimat

An International Journal of Infectious Diseases case report from the Military University Hospital Prague describes a 60-year-old woman with chronic MRSA vertebral osteomyelitis and non-removable spinal instrumentation who received 35 intrav

Perfilyev and colleagues analysed 3.6 million bacterial genome assemblies from 1,226 species in Nature Microbiology and found 119,510 lytic phage genomes, which they call bacterial assembly-associated phage sequences, a roughly five-fold in

The transatlantic taskforce on antimicrobial resistance sets out research and regulatory priorities for phage therapy, including standardized susceptibility testing, experimental models, GMP and personalized-treatment criteria, and clearer

Silverman and colleagues used RIL-seq to map RNA–RNA interactions in Escherichia coli during phage lambda infection, reported in Molecular Cell, and identified a phage-encoded small RNA, PreS (phage replication enhancer sRNA), embedded in t